冷链仓储创新装备在河南鲁山精准扶贫中的应用分析

文章对鲁山县董周乡酥梨冷箱仓储扶贫试点应用项目的实施情况开展了专题调研,现将多功能模块化组合冷箱配套农产品市场经营策略精准促进鲁山县董周乡农村产业扶贫的实施情况做系统阐述。     

正压给水的主排水泵启动特性仿真分析研究

峰峰集团某矿排水系统在采用正压给水方式后主排水泵泵效明显提高,为了研究排水系统的启动特性,利用Flowmaster软件建立了离心泵串联系统模型。根据水力机械试验室的设备参数进行了仿真,分析了正压给水条件下主泵定量启动前由单泵和双泵组成的排水系统特性。在此基础上,得到了离心泵瞬时启动转速和功率的特性曲线。结果表明,在正压给水条件下,主泵启动功率的影响基本消除,主泵功率达到稳定值的时间缩短。提高了主排水泵的效率,提高了排水系统的综合效率。     

矿产资源供应能力评价的FAHP方法

为了合理地开发和利用资源，提高资源利用效率和矿业发展质量，科学论证与评价矿产资源供应能力，具有重要的理论意义和现实意义。采用定性定量相结合的模糊层次分析法（FAHP），构建了矿产资源的供应能力的评价模型，并以我国2006—2015年钴资源的历史数据为例，将资源禀赋、供需市场、政治环境作为评价准则，通过对评价指标进行分析，得到了钴资源供应能力的评价值均在1以下，并呈现逐年下降趋势。研究结果表明，我国钴资源的供应能力整体偏弱，今后几年钴资源供应能力仍会维持在较低水平。     

Analysis of Modified Nucleotide Aptamer Library Generated by Thermophilic DNA Polymerases

One of the pivotal steps in aptamer selection is the amplification of target-specific oligonucleotides by thermophilic DNA polymerases; it can be a challenging task if nucleic acids possessing modified nucleotides are to be amplified. Hence, the identification of compatible DNA polymerase and modified nucleotide pairs is necessary for effective selection of aptamers with unnatural nucleotides. We present an in-depth study of using 5-indolyl-AA-dUTP (TAdUTP) to generate oligonucleotide libraries for aptamer selection. We found that, among the eight studied DNA polymerases, only Vent(exo-) and KOD XL are capable of adapting TAdUTP, and that replacing dTTP did not have a significant effect on the productivity of KOD XL. We demonstrated that water-in-oil emulsion PCR is suitable for the generation of aptamer libraries of modified nucleotides. Finally, high-throughput sequence analysis showed that neither the error rate nor the PCR bias was significantly affected by using TAdUTP. In summary, we propose that KOD XL and TAdUTP could be effectively used for aptamer selection without distorting the sequence space of random oligonucleotide libraries.     

环氧丙烷产业现状及发展趋势

简述了全球及我国环氧丙烷产业的生产及供需现状;对环氧丙烷在聚醚多元醇、丙二醇/碳酸二甲酯、丙二醇醚及其酯类等领域的应用进行了分析;并对环氧丙烷生产技术进行了比较,提出了发展建议。     

Dynamic Stabilization of DNA Assembly by Using Pyrrole-Imidazole Polyamide

We used N-methylpyrrole (Py)-N-methylimidazole-(Im) polyamide as an exogenous agent to modulate the formation of DNA assemblies at specific double-stranded sequences. The concept was demonstrated on the hybridization chain reaction that forms linear DNA. Through a series of melting curve analyses, we demonstrated that the binding of Py−Im polyamide positively influenced both the HCR initiation and elongation steps. In particular, Py−Im polyamide was found to drastically stabilize the DNA duplex such that its thermal stability approached that of an equivalent hairpin structure. Also, the polyamide served as an anchor between hairpin pairs in the HCR assembly, thus improving the originally weak interstrand stability. We hope that these proof-of-concept results can inspire future use of Py−Im polyamide as a molecular tool to modulate the formation of DNA assemblies.     

Peanut FAD2 Genotype and Growing Location Interactions Significantly Affect the Level of Oleic Acid in Seeds

The level of oleic acid is an important parameter in determining seed nutritional quality and oil stability. The level of oleic acid in peanut is genetically controlled by a pair of fatty acid desaturase genes (FAD2A and FAD2B), but the environmental conditions of the production sites can also have a significant effect. To investigate the effect of gene and environment interaction, 45 accessions were grown at three locations for 2 years. Environmental data were collected; individual plants were genotyped with functional SNP markers from FAD2A and FAD2B; and seed level of oleic acid was determined by gas chromatography. Three FAD2A/FAD2B genotypes (448G/no insertion 442A, 448A/no insertion 442A, and 448A/insertion 442A) were identified and designated as G/W, A/W, and A/A, respectively. A/A genotype averaged the highest level of oleic acid (80.0%), followed by A/W (56.0%), and then G/W (40.7%). Analysis of gene and environment interaction revealed that oleic acid phenotype plasticity could be explained by the interaction of FAD2 genotype and photothermal time, which quantified environmental conditions. The A/W genotype was the most sensitive to photothermal time changes. The oleic acid plasticity revealed in this study would be useful for breeders, farmers, and product processors.     

溢出效应和网络贴合度扰动下的二级供应链网络广告投放策略分析

为研究溢出效应和网络贴合度扰动下,二级供应链的网络广告投放策略问题,以强势制造商和两个网络零售商组成的二级供应链为研究对象,考虑产品的网络贴合度和网络广告计费特征,构建基于溢出效应的期望需求模型,通过博弈论方法给出了完全不合作、合作、半合作、战略联盟等4种模式下的均衡解,并对以上4种均衡结果进行比较分析。研究结果表明:无论在何种模式下,制造商批发价格与产品的网络贴合度成反比;固定位网络广告投资额和网络广告投资总额与产品的网络贴合度成正比,固定位网络广告投资额与溢出效应成反比;合作状态下的利润总额通常情况下较低;当产品的网络贴合度超过一定阈值范围时,采用战略联盟使得供应链成员利润最大化。     

某800 t/d级浮法薄玻璃生产线项目建设的得失分析

总结了某800 t/d浮法薄玻璃生产线项目建设的教训与经验,通过项目过程中发生的真实案例,对项目建设中存在的不足进行了深入剖析,并就项目的方案制定、材料与供方选择、建设工期与质量控制等工作提出了改进建议。